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high resolution melt analysis hrma  (Bio-Rad)


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    Bio-Rad high resolution melt analysis hrma

    High Resolution Melt Analysis Hrma, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 451 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+resolution+melt+analysis+hrma/Precision+Melt+Analysis+Software/bio_rxiv__2025__11__26__690707-74-7-17
    Average 96 stars, based on 451 article reviews
    high resolution melt analysis hrma - by Bioz Stars, 2026-09
    96/100 stars

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    1) Product Images from "Functional Landscape of Zebrafish Gonadotropins and Receptors: A Comprehensive Genetic Analysis"

    Article Title: Functional Landscape of Zebrafish Gonadotropins and Receptors: A Comprehensive Genetic Analysis

    Journal: bioRxiv

    doi: 10.1101/2025.11.26.690707


    Figure Legend Snippet:

    Techniques Used:



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    Image Search Results


    Journal: bioRxiv

    Article Title: Functional Landscape of Zebrafish Gonadotropins and Receptors: A Comprehensive Genetic Analysis

    doi: 10.1101/2025.11.26.690707

    Figure Lengend Snippet:

    Article Snippet: The extracted genomic DNA was analyzed using High-Resolution Melt Analysis (HRMA) with the Precision Melt Analysis software (Bio-Rad Laboratories, Hercules, CA) to identify different genotypes based on melt curve differences [ ].

    Techniques:

    Development of a Zebrafish CRISPR/Cas9 pipeline to assess functional impact of DMD modifier candidates . (A) Adult fish heterozygous for dmd are intercrossed to generate embryos for the screen. Because DMD in zebrafish follows an autosomal recessive inheritance pattern, one-quarter of the intercross progeny are homozygous dmd mutants ( dmd-/- ), all of which are identifiable using birefringence imaging by 4 dpf due to the presence of muscle lesions. Homozygous wild-type ( dmd+/+ ) and heterozygous dmd ( dmd+/- ) fish are indistinguishable. Embryos are injected by the 1-cell stage with modifier-targeting CRISPR and Cas9 protein or raised as uninjected controls. All fish are assayed for DMD onset between 2-4 dpf using live birefringence imaging. At 4 dpf, all fish are fixed, birefringence imaged, and genotyped via HRMA. (B, B’) Representative examples of wild-type (WT, top) and dmd-/- mutant (bottom) fish imaged via birefringence at 4 dpf. The boxes in B are shown magnified in B’. Wild-type fish show strong, uniform birefringence, whereas dmd mutant fish have variably-sized non-birefringence patches indicating dystrophic lesions (white arrows). Fish containing one or more lesion, regardless of lesion size or number, are scored as dystrophic in the onset assay.

    Journal: bioRxiv

    Article Title: Validation of Duchenne muscular dystrophy candidate modifiers using a CRISPR-Cas9-based approach in zebrafish

    doi: 10.1101/2025.05.20.655139

    Figure Lengend Snippet: Development of a Zebrafish CRISPR/Cas9 pipeline to assess functional impact of DMD modifier candidates . (A) Adult fish heterozygous for dmd are intercrossed to generate embryos for the screen. Because DMD in zebrafish follows an autosomal recessive inheritance pattern, one-quarter of the intercross progeny are homozygous dmd mutants ( dmd-/- ), all of which are identifiable using birefringence imaging by 4 dpf due to the presence of muscle lesions. Homozygous wild-type ( dmd+/+ ) and heterozygous dmd ( dmd+/- ) fish are indistinguishable. Embryos are injected by the 1-cell stage with modifier-targeting CRISPR and Cas9 protein or raised as uninjected controls. All fish are assayed for DMD onset between 2-4 dpf using live birefringence imaging. At 4 dpf, all fish are fixed, birefringence imaged, and genotyped via HRMA. (B, B’) Representative examples of wild-type (WT, top) and dmd-/- mutant (bottom) fish imaged via birefringence at 4 dpf. The boxes in B are shown magnified in B’. Wild-type fish show strong, uniform birefringence, whereas dmd mutant fish have variably-sized non-birefringence patches indicating dystrophic lesions (white arrows). Fish containing one or more lesion, regardless of lesion size or number, are scored as dystrophic in the onset assay.

    Article Snippet: DNA was extracted from adult fin tissue or whole larvae as described above and 1ul was used as template in a 10ul HRMA reaction (BioRad 172-5112) in a CFX Duet Real-Time PCR System (#12016265) and analyzed using BioRad Precision Melt Analysis Software.

    Techniques: CRISPR, Functional Assay, Imaging, Injection, Mutagenesis